This site is devoted to mathematics and its applications. Created and run by Peter Saveliev.

# Particle statistics with calibration

The task for Pixcavator: "What I need it for the software to analyse the attached image in order to get an area for each particle. I compare the area with a standard 2mm square that is included in the image. I then take the values and create a histogram showing the size distribution of the particles."

I analyzed the image with these settings: 20, 10, 0, 69, 0, 255. The image is below.

I unmarked the light objects and saved the spreadsheet. Then I did some data processing with Excel. First I located the square, line #3248. Its area is 211, so:

211 pixels = 2 mm * 2 mm = 4 sq mm and 1 pixel = 4 / 211 sq mm = .019 sq mm.

I used the last number as the calibration factor in the other spreadsheet. It contains the areas in mm. It also contains the histogram of distribution of the area. I think the results make sense.