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Mobility and distribution of chlorophyll proteins

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Freeze-fracture electron micrographs from intact spinach chloroplasts.jpg

A research paper that uses Pixcavator:

Visualizing the mobility and distribution of chlorophyll proteins in higher plant thylakoid membranes: effects of photoinhibition and protein phosphorylation by Tomasz K. Goral, Matthew P. Johnson, Anthony P.R. Brain, Helmut Kirchhoff, Alexander V. Ruban and Conrad W. Mullineaux (School of Biological and Chemical Sciences, Queen Mary University of London; Centre for Ultrastructural Imaging, Kings College, University of London; and Institute of Biological Chemistry, Washington State University), link, published in The Plant Journal, Volume 62, Issue 6, pages 948–959, June 2010.

From the paper:

"The diffusion of proteins in chloroplast thylakoid membranes is believed to be important for processes including the photosystem-II repair cycle and the regulation of light harvesting. ... The PSII particle average density and distance measurements (n » 2000) were conducted using PIXCAVATOR IA 4.2 (Intelligent Perception, and the Delaunay Voronoi plug-in of the IMAGEJ software. Measurements of PSII particle sizes were carried out with IMAGEPRO PLUS software."

Some details of image analysis: Membranes containing proteins.

See other examples of image analysis.