Measuring seedling area

Q: We need to "measure all the seedlings area (by dividing by the number of them I can get indication to their area and structure, and other parameters I can get)... The area covered is 80*80cm."

The screenshot shows the results of my experiment. Here the red contours surround the darker areas (vegetation) and green surround the lighter areas inside. So, the total coverage is 60 - 9 = 51%. Unfortunately, I was unable to separate the seedlings from the rest of the vegetation.

There is also work with ecological researchers to measure vegetation coverage but mostly with horizontal shots.

Other examples of image analysis