Paper that uses Pixcavator:
Down-regulation of CXCR4 and CD62L in Chronic Lymphocytic Leukemia Cells Is Triggered by B-Cell Receptor Ligation and Associated with Progressive Disease  by Amalia Vlad, Pierre-Antoine Deglesne, Re´mi Letestu, Ste´phane Saint-Georges, Nathalie Chevallier, Fanny Baran-Marszak, Nadine Varin-Blank, Florence Ajchenbaum-Cymbalista, and Dominique Ledoux (Institut National de la Sante´ et de la Recherche Me´dicale-Universite´ Paris 13, Service d’he´matologie biologique, Hoˆpital Avicenne, Bobigny, France). Published in Cancer Research 69, 6387, August 15, 2009.
From the paper:
"Progressive cases of B-cell chronic lymphocytic leukemia (CLL) are frequently associated with lymphadenopathy, highlighting a critical role for signals emanating from the tumor environment in the accumulation of malignant B cells."
"BCR-stimulated and unstimulated fluorescent cells were mixed in RPMI 1640/10% FCS and added together onto the endothelial cell layer. After incubation for 2 h at 37jC, the nonadherent CLL cells were washed off. Remaining adherent cells were fixed, and 10 fields from duplicate chamber slides (average of 500 cells/field) were photographed under fluorescent microscope. Red and green fluorescence were separately quantified using the Pixcavator IA 3.3 software (Intelligence Perception Co.)."