Counting stained DNA
Q: we need to "count cell/dna etc from still microscopic image... Here we stained DNA ...S-phase are in red and G-phase is in Blue. We want to count how many red are there and how many blue are there."
Now the red cells are so bright that in the blue channel they are also present. So, here you see both red and blue and have to subtract:
299 - 71 = 228 of the blue ones.
Image processing can help too - I simply removed the red using Photoshop Elements.
The total was 204:
One day you'll be able to use the true color analysis.